Brochure of the Tristar 3 and Tristar 5 Multimode Microplate Readers
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Highlights
Detection Technologies
The Tristar 5 Multimode Microplate Reader provides you with application flexibility for today, tomorrow, and beyond to master your changing plate reading applications. The system supports the following wide range of detection modes:
- Absorbance (UV/VIS)
- Fluorescence Intensity (including FRET)
- Luminescence (including BRET and BRET2)
- Time Resolved Fluorescence (TRF)
- Time Resolved FRET (TR-FRET / HTRF®)
- Fluorescence Polarization
- AlphaScreen®
FlexTec Optics – when flexibility AND sensitivity count
The Tristar 5 system is equiped with FlexTec Optics, offering you the best of two worlds – benefit from the flexibility to easily select a discrete wavelength and perform spectral scans for both, excitation and emission using its built-in monochromator technology. Or optimize your assays´ sensitivity by utilizing the system filter sliders for excitation and emission. Or mix both technologies if required: the Tristar 5 delivers both, flexibility and sensitivity in a single system.
The Tristar 5 employs up to two double monochromators providing blocking properties needed in sensitive fluorescence assays. Both monochromators are equipped with software driven continuous bandwidth variation to optimise the instrument for the specific demands of different assay conditions.
Berthold´s flexible monochromator technology offers you variable bandwidths from 4-12 nm in excitation and 8 – 22 nm for emission, selectable in 1 nm increments.
Broad wavelength range selection from UV through the visible range
From UV absorbance for DNA quantification to measurements in the far red or NIR (in selected configurations), the Tristar 5 is ready for any assay.
Advanced bottom reading technology - enhanced cell-based assay performance
The Tristar 5 enables you to read both, from below as well as from the top with high-performance. The advanced bottom-reading technology of the system can be easily selected via the software to help you analyse e.g. adherent cells and obtain best sensitivity and a superior signal-to-background ratio for these applications.
ONE-4-ALL Optics - uncompromised performance
Our patented ONE-4-ALL optics have been optimised to combine the stability and user-friendliness of a multimodal optical system with the sensitivity and versatility of dedicated optical devices.
ONE-4-ALL optics work for both filter- and monochromator-based applications, so there´s never a compromise in performance in any mode.
High-performance filter system - when sensitivity counts
The quick-change filter technology of the Tristar series provides you with the flexibility required to meet your application needs: up to 40 different excitation and emission filters can be easily mounted on exchangeable filter slides.
The filters are characterized by high transmissions properties which can be up to 25-fold that of monochromators. Technologies like Time-Resolved Fluorescence (TRF) can be measured more efficiently with filters.
Furthermore, filters with a large bandwidth are available to analyze fluorophores with wide spectra and all luminescence-based assay requiring to use filters, e.g. BRET, BRET2 or NanoBRET™ assays.
Ergonomic design - supporting your workflows
The ergonomic design of the system provides full front access for all key operations (e.g. plate loading, filter change and connecting reagents). Reagent vials can be stored in an integrated front compartment, providing easy access and visibility. It contains a removable trough that can be filled with water or ice to keep all reagents cooled. Different reagent tube holders are available to ensure secure handling of valuable reagents. The system has a flat surface on its top, providing enough space to put down a laptop.
Powerful crosstalk control – reliable results
Do you often see a few high-intensity samples surrounded by wells of low-intensity when running your samples? Then you´re potentially affected by a phenomenon called crosstalk, that can produce wrong results and, hence, wrong conclusions. The crosstalk reduction design of the Tristar 5 does almost eliminate this problem: with a crosstalk of 10-6, you never have to worry about the position of your high-intensity samples in your plate.
JET injectors: highest accuracy, speed and cell-friendliness
The Tristar 5 can be equipped with up to 3 JET injectors with variable volumes in pre-measurement or measurement position. The ultra-fast injection speed of JET injectors eliminates any delay between injection and measurement and enables measurement of fast reactions, e.g. flash luminescence.
Berthold Technologies' JET injectors use Teflon bellows for accurate and fast injections and guarantee most efficient mixing as well as extreme longevity.
- Highest accuracy and reproducibility - 98 % accuracy over the entire volume range
- Low reagent consumption - Low dead volume. Recovers up to 60 % of the reagent in the injector line. A single priming sequence is sufficient to achieve a 99 % homogeneous mix at the tip.
- Low maintenance costs – friction-free operation enables more than 3 million injections without mechanical failure.
- Optimal performance in flash-kinetics - ultra-fast injection enables the measurement of the first 150 ms of a kinetic assay (e.g. Fura-2 or Acridiniumester-assays).
- Worry-free injection of cell suspensions - negligible shear forces ideal when working with living cells, e.g. in Aequorin-based calcium assays
Convenient Software options
Our microplate readers are powered by LightCompass®, a flexible software enabling easy data acquisition, analysis and reporting using Berthold Technologies' instruments. It has been optimized for different microplate and tube formats. LightCompass® features all the functions you can expect from an advanced scientific software, such as flexible creation of measurement protocols, curve fitting, qualitative analysis and compliance with FDA 21 CFR Part 11.
LightCompass® is available in 3 different versions: Basic, Plus and Professional. Just pick the version that better suits your research needs.
Validation tools, Service & Support
For many laboratories, validation, qualification and ensuring compliance with a number of GMP and GLP requirements
is essential. We provide a number of tools and services to help you ensure that your system is running at
peak performance.
Validation Tools
We provide a variety of tools to help you check and confirm the performance of your products over time.
Service
Berthold Expert Services provide a team of dedicated and factory-trained engineers and experts to optimize your productivity. We and our local partners are always at your disposal.
Support
Our team of technical support scientists is your partner to overcome the unique challenges your application brings. Contact our team to discuss your assay or experiment design, data analysis questions, troubleshooting and more.
Featured Application Notes
DNA quantification using Quant-iT™ Picogreen dsDNA
The combination of the Quant-iT™ Picogreen dsDNA assay and the Tristar microplate readers offers a fast, simple and accurate way to quantify DNA in your samples.
Study Protein-Protein interactions with NanoBRET™
This publication confirms the suitability of the Tristar microplate readers to measure the NanoBRET™ assay, which is an improvement of the BRET technology resulting in increased signal and lower background.
Dual-Luciferase Reporter® (DLR™) Assay with Tristar
The Dual-Luciferase Reporter® (DLR™) Assay System from Promega is a popular commercial assay using firefly luciferase and renilla luciferase as reporters. Promega’s DLR™ assay had been validated on the Tristar 5 multimode microplate reader.
Tristar family Application Notes
Some of the following Application Notes were created with earlier instrument versions of the Tristar system family, but can be transferred 1:1 to the new Tristar systems.
QUANTIFYING DNA WITH THE QUANT-IT™ PICOGREEN® dsDNA KIT AND BERTHOLD TRISTAR MULTIMODE READERS The Quant-iT™ PicoGreen® dsDNA reagent is specific for dsDNA and is suitable for microplate readers. In combination with the Tristar 3 and Tristar 5 Multimode Microplate Readers, it allows the specific quantification of dsDNA in 96-well plates achieving a limit of detection below 0.1 pg/µL.
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MEASURING HTRF® ASSAYS WITH THE TRISTAR 5 MULTIMODE READER In this application note, the performance of the Tristar 5 multimode microplate reader is tested in 4 different HTRF® assays: TNFα and cAMP Gs HiRange (europium donor/red acceptor), IP-One Gq (Terbium donor/red acceptor) and cAMP HTPlex (Terbium donor/green acceptor). We conclude that the Tristar 5 performs well in all of them, as all required performance indicators are met.
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DLR™ VALIDATION OF THE TRISTAR 3 Validation of the Tristar 3 with the Dual-Luciferase® Reporter System from Promega.
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Transcreener FI ADP2 assay with the Tristar 5 Validation of the Tristar 5 Multimode Microplate Reader with the Transcreener® ADP2 FI Assay
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DLR™ VALIDATION OF THE TRISTAR 5 Validation of the Tristar 5 for the Dual-Luciferase® Reporter System from Promega.
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MONITORING MOLECULAR INTERACTIONS USING THE PROMEGA NANOBRET™ PROTEIN:PROTEIN INTERACTION SYSTEM AND THE TRISTAR MULTIMODE MICROPLATE READERS In this Application Note results confirm that Tristar Multimode Readers are suitable devices for use with the Promega NanoBRET™ Protein:Protein Interaction System.
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Transcreener® Fluorescence Intensity Assay with the TriStar² S The compatibility of the Transcreener® Fluorescence Intensity Assay Kit for ADP is tested with the TriStar² S LB 942 Multimode Microplate Reader.
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Transcreener® GDP TR-FRET Red Assay with TriStar² S Validation of the TriStar² S Multimode Microplate Reader with the Transcreener® GDP TR-FRET Red assay
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Screening of GPCR antagonists using Ca2+ measurements and the TriStar² S 3 commercially available compounds are screened for P2Y2 receptor inhibition using the calcium mobilisation assay with two different fluorescent dyes measured on a TriStar² LB 942 multimode microplate reader.
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Monitoring circadian gene expression in micro-algae using the TriStar² We report how the Berthold TriStar² multimode microplate reader equipped with luminescence module, in combination with an external LED illumination adaptor, can be used to image circadian rhythms in the micro algae Ostreococcus tauri.
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