Brochure of the Tristar 3 and Tristar 5 Multimode Microplate Readers
PDF | 983.0 KB
Download nowThe Tristar 3 Multimode Microplate Reader provides you with application flexibility for today, tomorrow, and beyond to master your changing plate reading applications:
From UV absorbance for DNA quantification to measurements in the far red or NIR (in selected configurations), the Tristar 3 is ready for any assay and greatly improves the wavelength range of the older TriStar².
All Tristar 3 preconfigured models have the features you need to perform BRET assays: best-in-class luminescence sensitivity and a high quality filter system. To start working with BRET, just order the upgrade for your specific assay: BRET1, BRET2, NanoBRET™ or others.
Our patented ONE-4-ALL optics have been optimised to combine the stability and user-friendliness of a multimodal optical system with the sensitivity and versatility of dedicated optical devices.
ONE-4-ALL optics work for both filter- and monochromator-based applications, so there´s never a compromise in performance in any mode.
The quick-change filter technology of the Tristar series provides you with the flexibility required to meet your application needs: up to 40 different excitation and emission filters can be easily mounted on exchangeable filter slides.
The filters are characterized by high transmissions properties which can be up to 25-fold that of monochromators. Technologies like Time-Resolved Fluorescence (TRF) can be measured more efficiently with filters.
Furthermore, filters with a large bandwidth are available to analyze fluorophores with wide spectra and all luminescence-based assay requiring to use filters, e.g. BRET, BRET2 and NanoBRET™ assays.
The ergonomic design of the system provides full front access for all key operations (e.g. plate loading, filter change and connecting reagents). Reagent vials can be stored in an integrated front compartment, providing easy access and visibility. It contains a removable trough that can be filled with water or ice to keep all reagents cooled. Different reagent tube holders are available to ensure secure handling of valuable reagents. The system has a flat surface on its top, providing enough space to put down a laptop.
Do you often see a few high-intensity samples surrounded by wells of low-intensity when running your samples? Then you´re potentially affected by a phenomenon called crosstalk, that can produce wrong results and, hence, wrong conclusions. The crosstalk reduction design of the Tristar 3 does almost eliminate this problem: with a crosstalk of 10-6, you never have to worry about the position of your high-intensity samples in your plate.
The Tristar 3 can be equipped with up to 3 JET injectors with variable volumes in pre-measurement or measurement position. The ultra-fast injection speed of JET injectors eliminates any delay between injection and measurement and enables measurement of fast reactions, e.g. flash luminescence.
Berthold Technologies' JET injectors use Teflon bellows for accurate and fast injections and guarantee most efficient mixing as well as extreme longevity.
Our microplate readers are powered by LightCompass®, a flexible software enabling easy data acquisition, analysis and reporting using Berthold Technologies' instruments. It has been optimized for different microplate and tube formats. LightCompass® features all the functions you can expect from an advanced scientific software, such as flexible creation of measurement protocols, curve fitting, qualitative analysis and compliance with FDA 21 CFR Part 11.
LightCompass® is available in 3 different versions: Basic, Plus and Professional. Just pick the version that better suits your research needs.
For many laboratories, validation, qualification and ensuring compliance with a number of GMP and GLP requirements is essential. We provide a number of tools and services to help you ensure that your system is running at peak performance.
We provide a variety of tools to help you check and confirm the performance of your products over time.
Berthold Expert Services provide a team of dedicated and factory-trained engineers and experts to optimize your productivity. We and our local partners are always at your disposal.
Our team of technical support scientists is your partner to overcome the unique challenges your application brings. Contact our team to discuss your assay or experiment design, data analysis questions, troubleshooting and more.
The combination of the Quant-iT™ Picogreen® dsDNA assay and the Tristar microplate readers offers a fast, simple and accurate way to quantify DNA in your samples.
This publication confirms the suitability of the Tristar microplate readers to measure the NanoBRET™ assay, which is an improvement of the BRET technology resulting in increased signal and lower background.
The Dual-Luciferase Reporter® (DLR™) Assay System from Promega is a popular commercial assay using firefly luciferase and renilla luciferase as reporters. Promega’s DLR assay had been validated on the Tristar 3 multimode microplate reader.
Some of the following Application Notes were created with earlier instrument versions of the Tristar system family, but can be transferred 1:1 to the new Tristar systems.
QUANTIFYING DNA WITH THE QUANT-IT™ PICOGREEN® dsDNA KIT AND BERTHOLD TRISTAR MULTIMODE READERS The Quant-iT™ PicoGreen® dsDNA reagent is specific for dsDNA and is suitable for microplate readers. In combination with the Tristar 3 and Tristar 5 Multimode Microplate Readers, it allows the specific quantification of dsDNA in 96-well plates achieving a limit of detection below 0.1 pg/µL.
PDF | 459.4 KB
MEASURING HTRF® ASSAYS WITH THE TRISTAR 5 MULTIMODE READER In this application note, the performance of the Tristar 5 multimode microplate reader is tested in 4 different HTRF® assays: TNFα and cAMP Gs HiRange (europium donor/red acceptor), IP-One Gq (Terbium donor/red acceptor) and cAMP HTPlex (Terbium donor/green acceptor). We conclude that the Tristar 5 performs well in all of them, as all required performance indicators are met.
PDF | 463.0 KB
DLR™ VALIDATION OF THE TRISTAR 3 Validation of the Tristar 3 with the Dual-Luciferase® Reporter System from Promega.
PDF | 283.1 KB
Transcreener FI ADP2 assay with the Tristar 5 Validation of the Tristar 5 Multimode Microplate Reader with the Transcreener® ADP2 FI Assay
PDF | 365.3 KB
DLR™ VALIDATION OF THE TRISTAR 5 Validation of the Tristar 5 for the Dual-Luciferase® Reporter System from Promega.
PDF | 295.5 KB
MONITORING MOLECULAR INTERACTIONS USING THE PROMEGA NANOBRET™ PROTEIN:PROTEIN INTERACTION SYSTEM AND THE TRISTAR MULTIMODE MICROPLATE READERS In this Application Note results confirm that Tristar Multimode Readers are suitable devices for use with the Promega NanoBRET™ Protein:Protein Interaction System.
PDF | 375.3 KB
Transcreener® Fluorescence Intensity Assay with the TriStar² S The compatibility of the Transcreener® Fluorescence Intensity Assay Kit for ADP is tested with the TriStar² S LB 942 Multimode Microplate Reader.
PDF | 1.0 MB
Transcreener® GDP TR-FRET Red Assay with TriStar² S Validation of the TriStar² S Multimode Microplate Reader with the Transcreener® GDP TR-FRET Red assay
PDF | 934.0 KB
Screening of GPCR antagonists using Ca2+ measurements and the TriStar² S 3 commercially available compounds are screened for P2Y2 receptor inhibition using the calcium mobilisation assay with two different fluorescent dyes measured on a TriStar² LB 942 multimode microplate reader.
PDF | 1,009.5 KB
Monitoring circadian gene expression in micro-algae using the TriStar² We report how the Berthold TriStar² multimode microplate reader equipped with luminescence module, in combination with an external LED illumination adaptor, can be used to image circadian rhythms in the micro algae Ostreococcus tauri.
PDF | 675.1 KB
Detectors |
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Detection technologies |
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Measurement modes | Endpoint, Kinetics, Repeated, Spectral scan, Area scan (up to 100 x 100 points per well) |
Filters | High quality interference filters |
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Filter handling | Easy to change filter slides with up to 5 filters per slide, additional slides available |
Optical system | ONE-4-ALL optics |
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Light source | Xenon flash lamp |
Spectral range Absorbance | 200-1000 nm |
Spectral range Luminescence | Standard: 280-650 nm FL models: 280-850 nm |
Spectral range Fluorescence | Excitation: 200-1000 nm Emission, standard: 280-650 nm Emission, FL models: 280-850 nm |
Injectors | Up to 3 JET injectors with frictionless operation |
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Injector volume | 10-100 µL in 1 µL steps |
Injection speed | 200 – 440 μL/sec |
Injector accuracy | Better than 2% |
Injector precision | Better than 2% |
Microplate formats | 6- to 384-well |
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Plate height adjustment | 3 different frames depending on plate type |
Additional sample formats (optional) |
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Shaking | Linear, orbital, double orbital, with selectable speed and diameter |
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Temperature control | From ambient +5° to 45° C, includes additional cooling for the photomultiplier |
Gas connection | Yes (optional) |
Absorbance precision | Better than 0.6% |
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Absorbance accuracy | Better than 2% |
Luminescence sensitivity | <6 amol (0.03 pM) ATP/well (96-well plate) |
Fluorescence sensitivity | <7 amol Fluorescein/well (384-well small volume plate) |
Crosstalk | 10-6 (black plates) |
Dynamic range | >6 orders of magnitude 0-3.5 OD |
Interface | Windows software, connected to computer via USB |
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Operating system | Windows 10 |
Available software packages | LightCompass®: flexible software for data acquisition, analysis and reporting. |
Regulations | CE, NRTL |
Power Supply | 110-240 VAC ± 10% |
Operating Voltage | 24 VDC ± 5% |
Power consumption | 140 VA |
Temperature tolerance | Storage: 0-40° C Operation: 15-35° C |
Humidity tolerance | 10-80% non-condensing |
Altitude | max 2000 m (above sea level) |
Dimensions (W x D x H) | 391 x 470 x 344 mm |
Weight | approx. 20 kg |
Brochure of the Tristar 3 and Tristar 5 Multimode Microplate Readers
PDF | 983.0 KB
Download nowBrochure of the Tristar 3 Multimode Microplate Reader
PDF | 850.4 KB
Download nowValidation of the Tristar 3 with the Dual-Luciferase® Reporter System from…
EN | PDF | 283.1 KB
Download nowTristar multimode readers, in combination with the μDrop™ plate, enable the…
EN | PDF | 422.5 KB
Download nowIn this Application Note results confirm that Tristar Multimode Readers are…
PDF | 375.3 KB
Download nowThe Quant-iT™ PicoGreen® dsDNA reagent is specific for dsDNA and is suitable for…
EN | PDF | 459.4 KB
Download nowEN | PDF | 3.2 MB
Download nowEN | PDF | 1.3 MB
Download nowA compilation of scientific articles using Mithras or Tristar microplate readers…
PDF | 511.6 KB
Download nowEXE | 69.2 MB
Download nowBrochure of the Tristar 3 and Tristar 5 Multimode Microplate Readers
PDF | 983.0 KB
Download nowBrochure of the Tristar 3 Multimode Microplate Reader
PDF | 850.4 KB
Download nowValidation of the Tristar 3 with the Dual-Luciferase® Reporter System from…
EN | PDF | 283.1 KB
Download nowTristar multimode readers, in combination with the μDrop™ plate, enable the…
EN | PDF | 422.5 KB
Download nowIn this Application Note results confirm that Tristar Multimode Readers are…
PDF | 375.3 KB
Download nowThe Quant-iT™ PicoGreen® dsDNA reagent is specific for dsDNA and is suitable for…
EN | PDF | 459.4 KB
Download nowEN | PDF | 3.2 MB
Download nowEN | PDF | 1.3 MB
Download nowA compilation of scientific articles using Mithras or Tristar microplate readers…
PDF | 511.6 KB
Download nowEXE | 69.2 MB
Download nowThe Tristar 3 is a very recent instrument and thus not many papers using it have yet been published. Here you have a selection of recent articles.
Year | Authors | Journal | Title | Method |
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2024 | Zhangcheng Chen, Jing Yu, Huan Wang, Peiyu Xu, Luyu Fan, Fengxiu Sun, Sijie Huang, Pei Zhang, He Huang, Shuo Gu, Bowen Zhang Yue Zhou, Xiaobo Wan, Gang Pei, H Eric Xu, Jianjun Cheng, Sheng Wang | Cell | Flexible scaffold-based cheminformatics approach for polypharmacological drug design | BRET |
2023 | Nils Mülling, Lukas van de Sand, Kim Völk, Ulrich Wilhelm Aufderhorst, Mark van der Linden, Peter A. Horn, Andreas Kribben, Benjamin Wilde, Adalbert Krawczyk, Oliver Witzke, and Monika Lindemann | Infection | ELISA | |
2023 | Martin J. Raftery, Alexander Sebastian Franzén, Clarissa Radecke, Abdelhadi Boulifa, Günther Schönrich, Sebastian Stintzing, Jens-Uwe Blohmer, Writing – review & editing, Gabriele Pecher | International Journal of Molecular Sciences | Next Generation CD44v6-Specific CAR-NK Cells Effective against Triple Negative Breast Cancer | Nano-Glo© luciferase assay system |
2023 | Hannah J. Stage, Susanne Trappe, Katharina Söllig, Dagmar S. Trachsel, Katharina Kirsch, Cornelia Zieger, Roswitha Merle, Jörg R. Aschenbach, Heidrun Gehlen | Animals | Glycerol 3-Phosphate Dehydrogenase Assay | |
2023 | Sung-Bae Kim, Tadaomi Furuta, Genta Kamiya, Nobuo Kitada, Ramasamy Paulmurugan, Shojiro A. Maki | Sensors | Bright Molecular Strain Probe Templates for Reporting Protein–Protein Interactions | Luminescence of novel luciferases |
2022 | Ryotaro Nouda, Shohei Minami, Yuta Kanai, Takahiro Kawagishi, Jeffery A. Nurdin, Moeko Yamasaki, Ryusei Kuwata, Hiroshi Shimoda, Ken Maeda, and Takeshi Kobayashi | PNAS | Nano-Glo® HiBiT Lytic Detection System | |
2022 | Sinnberg T, Lichtensteiger C, Hill-Mündel K, Leischner C, Niessner H, Busch C, Renner O, Wyss N, Flatz L, Lauer UM, Hoelzle LE, Nohr D, Burkard M, Marongiu L, Venturelli S. | Antioxidants | Calcidiol ELISA | |
2022 | Long Cheng, Yan Wang, Jiawei Wang, Haibo Qin, Guangbiao Zhu, and Lingsong Tao | J Healthc Eng | MTS, ATP bioluminescent somatic cell assay kit |
You can find additional publications which have used older Tristar instruments in publication databases.
Please keep in mind that the list provided is not exhaustive, but it offers valuable examples of experiments performed with our instruments and published in peer-reviewed journals.
Tristar 3 Research 69173-10 | Tristar 3 Research Plus 69173-20 | Tristar 3 Research FL 69173-30 | Tristar 3 Research Plus FL 69173-40 | |
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Absorbance | ||||
Luminescence | ||||
Fluorescence (incl. FRET) up to 650 nm | ||||
Fluorescence (incl. FRET) up to 850 nm | ||||
BRET/BRET2 | optional | optional | optional | optional |
Temperature control | ||||
LightCompass Software |
Quant-iT™ and Picogreen® are registered trademarks of Thermo Fisher Scientific
NanoBRET™, Dual-Luciferase Reporter® and DLR™ are registered trademarks of Promega Corporation.