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Detection Modes

Detection ModesTristar 3Tristar 5ApolloCentroCentro XS
Absorbance (Vis)     
Absorbance (UV)  from 340 nm  
Fluorescence (Vis)     
Fluorescence (UV)     
FRET     
FP     
TRF     
HTRF®     
AlphaScreen®     
Glow Luminescence     
Flash Luminescence     
BRET     

Technical Features

Technical FeaturesTristar 3Tristar 5ApolloCentroCentro XS
Bottom Reading     
Monochromator     
Injectors   up to 3up to 3
Temperature control     
Shaking     
Gas connection     
Absorbance spectral scanning     
Fluorescence spectral scanning     
Luminescence spectral scanning     
96 well     
384 well     
6-48 well     
1536 well     

 

Assay Types

Assay TypesTristar 3Tristar 5ApolloCentroCentro XS
Endpoint assays     
Kinetic assays     
Well scanning     
Immunoassays     
ELISA     
Fluorescence immunoassays     
Luminescence immunoassays     

 

Protein Concentration

Protein ConcentrationTristar 3Tristar 5ApolloCentroCentro XS
Bradford, BCA, Lowry     
Protein (A280)     
Low volume absorbance (2 µL)     
NanoOrange®, CBQCA, Quant-iT™     

 

Nucleic Acid Concentration

Nucleic Acid ConcentrationTristar 3Tristar 5ApolloCentroCentro XS
DNA, RNA, oligos (A260)     
A260/A280     
Low volume DNA samples (2 µL)     

RiboGreen®, PicoGreen®, OliGreen®

     
Quant-iT™ RNA     

 

Reporter Genes

Reporter GenesTristar 3Tristar 5ApolloCentroCentro XS
Secreted Alkaline Phosphatase (SEAP)     
ß-Galactosidase (ß-Gal)     
ß-Glucuronidase (GUS)     
Luciferase     
Dual Luciferase     
GFP, CFP, YFP     

 

Cell Viability, Proliferation and Death

Cell Viability, Proliferation and Death

Tristar 3Tristar 5ApolloCentroCentro XS

MTT, XTT, Crystal Violet, SRB

     

Fluorescent Live/Dead kits, Alamar Blue

     

Calcein AM, CyQUANT®

     

SYTOX® Green, ethidium homodimer

     

 

Enzyme Assays

Enzyme AssaysTristar 3Tristar 5ApolloCentroCentro XS
ATP (luciferase)     
NADH (dehydrogenases)     
Phosphatases/Kinases activity     
Protease activity     

 

HTS Assays

HTS AssaysTristar 3Tristar 5ApolloCentroCentro XS

ABEL® Luminescent antioxidant test (Knight Scientific)

     

Adapta™ (Invitrogen)

     

AlphaScreen® (Perkin Elmer)

     

ATP bioluminescence

     

DELFIA® (Perkin Elmer)

     

DLR™ (Promega)

     

Dual-Glo™ (Promega)

     

GeneBLAzer™ (Invitrogen)

     

HitHunter® (DiscoveRx, luminescence)

     

HitHunter® (DiscoveRx, FP)

 *   

HTRF® (Cisbio)

     

Intracellular calcium (Ca2+ assay)

     

Ion Channel - VSP (Invitrogen)

     

LanthaScreen® (Invitrogen)

     

MycoAlert™ (Cambrex)

     

P450-Glo™ (Promega)

     

PKLight® (Cambrex)

     

PolarScreen™ (Invitrogen)

 *   

Predictor™ (Invitrogen)

     

Transcreener® FI (BellBrook Labs)

     

Transcreener® TR-FRET (BellBrook Labs)

     

Transcreener® FP (BellBrook Labs)

 *   

Z'-Lyte (Invitrogen)

     

* Not enought data. Please inquire.

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ABEL is registered trademark of Knight Scientific Limited.

Chroma-Glo, Kinase-Glo, DLR, Dual-Glo and P450-Glo are trademarks of Promega Corp.

MycoAlert and PK Light are trademarks of Cambrex.

HitHunter is a trademark of DiscoveRx.

Adapta, GeneBLAzer, Predictor, NanoOrange, PicoGreen, RiboGreen, OliGreen, Quant-iT, CBQCA, PolarScreen, LanthaScreen, Ion Channel VSP, Z'-Lyte, Alamar Blue, and Alexa Fluor are trademarks or registered trademarks of Thermo Fisher Scientific.

AlphaScreen, AlphaLISA, AlphaPlex, DELFIA, and LANCE are registered trademarks of Perkin Elmer Inc.

HTRF is a registered trademark of Cisbio International.

Transcreener is a patented technology of BellBrook Labs.

Cy Dyes are trademarks of GE Healthcare.