FlowStar² LB 514 - Berthold Technologies GmbH & Co.KG

Highlights

User friendly

The FlowStar² LB 514 is designed to be intuitive, easy to use and to get you up and running quickly. The intuitive touch screen interface makes it easy to get started and keep going. Results are displayed in both a graphical chromatogram and a numerical format, so you can start interpreting your results in real time while the run is still in progress.

The FlowStar² frees you from configuring parameters manually, thanks to the use of SmartCells: all measuring cells are equipped with a chip to automatically set the correct cell-specific system parameters. In addition, the software includes an extensive isotope library with predefined energy windows and cell usage options (¹⁴C, ³H, ³²P, ³³P, ³⁵S, ¹²⁸I, ¹¹¹In, ⁹⁹mTc…).

Easy integration into your HPLC system

The FlowStar² LB 514 makes integration easy to make sure everything works together to ensure consistent and reliable results. The integrated A/D converter records signals from external instruments (e.g. UV-detector) without requiring expensive additional hardware, while the USB port enables external control using RadioStar or other HPLC control software (e.g. Chromeleon™).

In addition, the dual analog output allows for the convenient integration into existing HPLC data systems.

The FlowStar² supports UHPLC and other LC technologies:

UHPLC
HPLC-MS (Mass Spectrometry)
HPLC-NMR (Nuclear Magnetic Resonance)

Safe and secure

The FlowStar² LB 514 demonstrates our commitment to providing everything you need for safe and easy integration into your lab environment.

Safety features:

Automatic HV shutdown for system safety.
Built-in leak detection to prevent the measuring chamber from damage caused by aggressive liquids. The system will shut down automatically in the event of cell leakage.

Security features:

On-board system performance tests with instrument-based history function.
Password-based multi user access control to protect instrument from unauthorized use.

Validation:

Several test cells are available to perform periodic system performance tests. These cells have been developed to meet GLP/GMP requirements and to guarantee a continuous system performance.

High sensitivity

The FlowStar² is the ultimate platform for high-sensitivity radio-HPLC flow detection. It uses random coincidence counting and luminescence subtraction for ultimate performance. In addition, the integrated spectrum scanning feature provides improved optimization of detector setup for each isotope.

Easy-to-use software for maximum productivity

The RadioStar software combines the ability to run sophisticated measurements with a user-friendly interface. A Chromeleon™ driver is available, Chromeleon™ is known as a very versatile chromatography software package supporting many different hardware solutions. The driver is fully certified, digitally signed by Thermo Scientific™ and runs with versions 6.x and 7.x.

Measuring cells specific to your needs

Choose from a wide range of available SmartCells to meet your application needs. These high sensitivity cells are equipped with a SmartChip to automatically set the correct cell-specific system parameters. The automatic shut-down feature of the system after removal of a cell from the detector provides additional safety.

The range of SmartCells include:

Liquid scintillation (admixture) cells
Solid scintillator cells
Gamma isotopes and Positron Emitter (PET) cells
Cells for preparative SFC
Microbore cells for UHPLC and UPLC™
Special Iodine Cells (¹²⁵I)
Cherenkov cells for high energy beta emitters

Radio HPLC Cell Configuration and Application Finder

Technical Details

Detection unit	State-of-the-art ultrasensitive dual 2“ PMT detection system with random coincidence counting and luminescence subtraction.
Display	Graphical touch screen with 320 x 240 pixel resolution.

Interface	USB port (B-type) RS-232 for pump control
Inputs	Start-, Stop- and Ready signal (TTL). 2 analog inputs (24 bit resolution), variable voltage (bipolar, max 2.5 V).
Outputs	Scintillator pump control (RS-232 and analog). 2 analog signal outputs 0-1 V (2,5 times oversampling) with 16-bit resolution. Waste valve and fraction collector control output (open collector output).

Interface

USB port (B-type)

RS-232 for pump control

Inputs

Start-, Stop- and Ready signal (TTL).

2 analog inputs (24 bit resolution), variable voltage (bipolar, max 2.5 V).

Outputs

Scintillator pump control (RS-232 and analog).

2 analog signal outputs 0-1 V (2,5 times oversampling) with 16-bit resolution.

Waste valve and fraction collector control output (open collector output).

Software	The following possibilities are available: Built-in software operated with touch screen External control and evaluation via RadioStar software External control and evaluation via Chromeleon™ driver.

Software

The following possibilities are available:

Built-in software operated with touch screen
External control and evaluation via RadioStar software
External control and evaluation via Chromeleon™ driver.

Power Supply	90-264 V, 50/60 Hz
Dimensions (W x D x H)	410 x 170 x 410 mm Horizontal and vertical operation possible
Weight	16 kg
Operating Temperature	10-35°C
Storage Temperature	5-40°C
Humidity	10-90% (non condensing)

Downloads

FlowStar² LB 514 Brochure

Brochure of the FlowStar² LB 514 Radio HPLC Detector

PDF | 5.1 MB

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FlowStar² LB 514 User Manual

EN | PDF | 5.1 MB

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Applications

Drug metabolism studies
Drug synthesis and purity
Toxicological studies
Purification of radio chemicals
Development and Quality Control of radio chemicals
Quality Control of radiolabelled antibodies and proteins

Publications

Here you have a selection of recent papers using FlowStar detectors.

Year	Authors	Journal	Title
2023	Anna Baitullina, Guilhem Claude, Suelen F. Sucena, Eda Nisli, Cedric Scholz, Punita Bhardwaj, Holger Amthauer, Winfried Brenner, Christopher Geppert, Christian Gorges, Ulrich Abram, Pedro Ivo da Silva Maia, and Sarah Spreckelmeyer	EJNMMI Radiopharmacy and Chemistry	Metallacages with 2,6-dipicolinoylbis(N,N-dialkylthioureas) as novel platforms in nuclear medicine for ⁶⁸Ga, ¹⁷⁷Lu and ¹⁹⁸Au
2023	Faustine d’Orchymont and Jason P. Holland	Communications Chemistry	Asymmetric rotaxanes as dual-modality supramolecular imaging agents for targeting cancer biomarkers
2022	Daniel F. Earley, Jose Esteban Flores, Amaury Guillou, and Jason P. Holland	Dalton Transactions	Photoactivatable bis(thiosemicarbazone) derivatives for copper-64 radiotracer synthesis
2021	Marion Berdal, Sébastien Gouard, Romain Eychenne, Séverine Marionneau-Lambot, Mikaël Croyal, Alain Faivre-Chauvet, Michel Chérel, Joëlle Gaschet, Jean-François Gestin and François Guérard	Chemical Science	Investigation on the reactivity of nucleophilic radiohalogens with arylboronic acids in water: access to an efficient single-step method for the radioiodination and astatination of antibodies
2021	Anthony Ku, Misaki Kondo, Zhongli Cai, Jalna Meens, Min Rong Li, Laurie Ailles, and Raymond M. Reilly	EJNMMI Radiopharm Chem	Dose predictions for [¹⁷⁷Lu]Lu-DOTA-panitumumab F(ab′)2 in NRG mice with HNSCC patient-derived tumour xenografts based on [⁶⁴Cu]Cu-DOTA-panitumumab F(ab′)2 – implications for a PET theranostic strategy
2021	Lara Garcia-Varela, Khaled Attia, John Carlo Sembrano, Olivier Jacquet, Inês F. Antunes, Chantal Kwizera, Ton J. Visser, Rudi A. J. O. Dierckx, Philip H. Elsinga, and Gert Luurtsema	EJNMMI Radiopharm Chem	A new approach to produce [¹⁸F]MC225 via one-step synthesis, a PET radiotracer for measuring P-gp function
2021	Daniel F. Earley, Amaury Guillou, Dion van der Born, Alex J. Poot, and Jason P. Holland	Molecules	Microfluidic Preparation of ⁸⁹Zr-Radiolabelled Proteins by Flow Photochemistry
2020	Jan Lennart von Hacht, Sarah Erdmann, Lars Niederstadt, Sonal Prasad, Asja Wagener, Samantha Exner, Nicola Beindorff, Winfried Brenner, Carsten Grötzinger	PLoS One	Increasing molar activity by HPLC purification improves ⁶⁸Ga-DOTA-NAPamide tumor accumulation in a B16/F1 melanoma xenograft model
2020	Livia Elena Chilug, Dana Niculae, Radu Anton Leonte, Alexandrina Nan, Rodica Turcu, Cosmin Mustaciosu, Radu Marian Serban, Vasile Lavric and Gina Manda	Molecules	Preclinical Evaluation of NHS-Activated Gold Nanoparticles Functionalized with Bombesin or Neurotensin-Like Peptides for Targeting Colon and Prostate Tumours
2019	Armand D. Anoman, María Flores-Tornero, Ruben M. Benstein, Samira Blau, Sara Rosa-Téllez, Andrea Bräutigam, Alisdair R. Fernie, Jesús Muñoz-Bertomeu, Sören Schilasky, Andreas J. Meyer, Stanislav Kopriva, Juan Segura, Stephan Krueger and Roc Rosa	Plant Physiology	Deficiency in the Phosphorylated Pathway of Serine Biosynthesis Perturbs Sulfur Assimilation
2019	Elly L. van der Veen, Inês F. Antunes, Petra Maarsingh, Janet Hessels-Scheper, Rolf Zijlma, Hendrikus H. Boersma, Annelies Jorritsma-Smit, Geke A. P. Hospers, Elisabeth G. E. de Vries, Marjolijn N. Lub-de Hooge and Erik F. J. de Vries	EJNMMI Radiopharmacy and Chemistry	Clinical-grade N-(4-[¹⁸F]fluorobenzoyl)-interleukin-2 for PET imaging of activated T-cells in humans

Additional Publications

You can find additional publications which have used FlowStar detectors in publication databases.

Please keep in mind that the list provided is not exhaustive, but it offers valuable examples of experiments performed with our instruments and published in peer-reviewed journals.

Search in Google Scholar

Accessories

Liquid Scintillator Pump LB 5037

Flow cells for Radio HPLC

QC HPLC Test Cells

Even more tools to ease your workflow

Static mixer

To improve the mixing of the eluate with the liquid scintillator.

Splitters

To split the eluate before mixing with scintillator, allowing part of the eluate to be diverted to a fraction collector.

Waste valve

Since only part of the eluate is radioactive waste, a waste valve can ensure that only the radioactive part is transferred to a special waste container.

FlowStar² Detector for Radio HPLC

Maximum flexibility, outstanding sensitivity