Fluorescence Resonance Energy Transfer (FRET) is a physical phenomenon, that requires close proximity of a donor molecule and an acceptor molecule (1-10 nm typically). As a result of excitation light that only the donor can absorb, the donor dye changes into an excited state and transfers energy without the emission of a photon to the acceptor dye. If the acceptor dye is fluorescent, it will emit light, typically at a much longer wavelength than the donor dye can emit itself. Today, FRET techniques are getting employed in a wide variety of applications, such as receptor/ligand studies, membrane fusion assays, fluorogenic protease assays or immunoassays.
The diversity of methods and fluorophores available makes flexibility in fluorescence detection a must. Our microplate readers enable you to not just detect Fluorescence Intensity (FI) but other assay technologies like FRET, TRF, HTRF® or Fluorescence Polarization (FP).
Need help selecting the Fluorescence Reader that best fits your research needs?
Wait no more, our specialists will be glad to help you!