Brochure of the NightSHADE evo LB 985N In Vivo Plant Imaging System
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Flexible optical setup and exquisite sensitivity
The NightSHADE evo uses an ultra-sensitive back-illuminated (backlit) CCD camera with high quantum efficiency over the entire visible to near-infrared spectrum. Efficient cooling to a delta of -100°C ensures the lowest background and highest sensitivity for long exposure times. The camera can be mounted either on top of the instrument to take images from above or laterally for side view images enabling the seedlings to maintain upright in their natural vertical orientation.
Control key environmental conditions
The NightSHADE evo helps creating a standardized environment for your experiment.
- Temperature control – temperature-controlled base plate to keep the temperature stable at user defined settings between 15 and 30 °C.
- Daylight simulation – 2 LED panels with 4 different colors each. LEDs are individually tunable in intensity and duration to simulate daylight with both, spectral and intensity gradients.
- Humidity control – the system can be placed into any appropriate environmental chamber.
A modular system tailored to your workflow
The NightSHADE evo provides a light-tight imaging chamber that you can set up according to your experimental needs:
- Light-tight ports are available for the introduction of lightguides, cables or tubing, e.g. to water plants inside the chamber.
- You are able to choose between different excitation devices: Ringlight for single microplates or dishes, dual gooseneck for spot illumination of dedicated areas, or 4-fold floodlight for uniform illumination of large areas.
- Several sample tables are available: the turntable can be equipped with different sample holders, e.g. to analyze square Petri dishes in upright position for unattended processing of multiple samples. The anti-condensation table for up to 9 Petri dishes features temperature control in combination with a constant, circular air flow above the Petri dishes, preventing condensation in the lids for crystal clear imaging.
Powerful and intuitive indiGO™ software
The user-friendly IndiGO™ software controls the instrument and makes image processing easy. It provides the following innovative features:
- Easy wavelength management: dedicated filters for both, excitation and emission are selected via a filter inventory that provides you with information about the filter properties. The software provides the option to display up to 10 different wavelength (colors) in a single experiment. This way, multiple events can be located simultaneously.
Daylight simulation: a set of 2 LED panels, 4 colors each can be mounted at different heights inside the imaging chamber for daylight simulation. With IndiGO™ software you can control intensity and duration of each color individually to provide homogeneous illumination up to 1,800 μE or 15,000 Lux.
- Other features: optional 21 CRF part 11 compliance, animal alignment and digital mouse atlas alignment option.
Applications
The NightSHADE evo In Vivo Imaging System for plants is a very flexible instrument, and its range of applications is really broad. Some of the most popular ones are:
- Protein-Protein Interaction
- Circadian rhythms
- Stress monitoring
- Pathogen monitoring
Featured Application Notes
Plant in vivo imaging eBook
Improve your plant imaging skills and tackle common challenges with this expert guide into the latest techniques, technologies and applications, including circadian rhythm research and pathogen and stress monitoring.
Improved experimental setup for analysis or circadian rhythms
Learn more about an experimental setup that improves data quality, reduces luminescence variation between replicates and highly correlates with modelling predictions.
Plant pathogen monitoring with NightSHADE
See how to combine imaging of the prompt fluorescence of a transgenic pathogen with imaging of the delayed fluorescence of chlorophyll to follow the infection.
Camera
The NightShade uses an ultrasensitive CCD camera with high quantum efficiency over the entire visible to near-infrared spectrum
Side camera position
The camera can be mounted laterally for side view images, enabling you to maintain seedlings upright in their natural vertical orientation.
Turntables
Several sample tables are available from a turntable which can be equipped with different sample holders, to an anti-condensation table.
Daylight simulation
2 LED panels with 4 different colors each. LEDs are individually tunable in intensity and duration to simulate daylight with both, spectral and intensity gradients.