Brochure of the Centro and Centro XS microplate luminometers
PDF | 461.4 KB
Download nowThe Centro supports all basic luminescence technologies including:
Thanks to a stringent selection of photomultipliers working in photon counting mode and more than 40 years of experience building luminometers, the Centro provides highly sensitive luminescence detection: less than 5 amol ATP/well (25 fM) can be detected reliably. In the Centro XS, optics have been optimized to increase its sensitivity even further: below 1.8 amol ATP/well (9 fM).
Do you often see a few high-intensity samples surrounded by wells of low-intensity when running your samples? Then you´re potentially affected by a phenomenon called crosstalk, that can produce wrong results and, hence, wrong conclusions. The crosstalk reduction design of the Centro does almost eliminate this problem: with a crosstalk of 10-6, you never have to worry about the position of your high-intensity samples in your plate.
Thanks to its built-in waste pump, you don’t need to worry about loading microplates, waste containers or removal of injector tips for washing and filling the reagent lines; the built-in waste pump takes care of the safe removal of excess liquids.
The Centro can be equipped with up to 3 JET injectors with variable volumes in pre-measurement or measurement position*. The ultra-fast injection speed of JET injectors eliminates any delay between injection and measurement and enables measurement of fast reactions, e.g. flash luminescence.
Berthold Technologies' JET injectors use Teflon bellows for accurate and fast injections and guarantee most efficient mixing as well as extreme longevity.
* All injectors in pre-measurement position in the Centro XS.
Our microplate readers are powered by LightCompass®, a flexible software enabling easy data acquisition, analysis and reporting using Berthold Technologies' instruments. It has been optimized for different microplate and tube formats. LightCompass® features all the functions you can expect from an advanced scientific software, such as flexible creation of measurement protocols, curve fitting, qualitative analysis and compliance with FDA 21 CFR Part 11.
LightCompass® is available in 3 different versions: Basic, Plus and Professional. Just pick the version that better suits your research needs.
The Dual-Luciferase Reporter® (DLR™) Assay System from Promega is a popular commercial assay using firefly luciferase and renilla luciferase as reporters and has excellent performance on the Centro Luminometer.
The MycoAlert® and MycoAlert® PLUS Mycoplasma Detection Kits by Lonza, in combination with the Centro Microplate Luminometer, provide a fast, convenient, and reliable way to detect mycoplasma contamination.
Detectors | Low-noise photomultiplier tube in single photon counting mode |
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Detection technologies | Luminescence |
Measurement modes | Endpoint, Kinetics, Repeated, Area scan (up to 100 x 100 points per well) |
Spectral range luminescence | 340-630 nm |
Injectors | Up to 3 JET injectors with frictionless operation |
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Injector volume | 10-100 µL in 1 µL steps |
Injector accuracy | Better than 2% |
Injector precision | Better than 2% |
Microplate formats | 96- and 384*-well |
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*Centro XS only
Shaking | Linear, orbital, double orbital, with selectable speed and diameter |
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Temperature control | From ambient +5° to 45° C (not available in Centro XS) |
Luminescence sensitivity | Centro: <5 amol (25 fM) ATP/well (96-well plate) Centro XS: <1.8 amol (9 fM) ATP/well (96-well plate) |
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Crosstalk | 10-6 |
Dynamic range | >6 orders of magnitude |
Interface | Windows software, connected to computer via USB |
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Operating system | Windows 10 |
Available software packages | LightCompass®: flexible software for data acquisition, analysis and reporting. |
Regulations | CE, NRTL |
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Power Supply | 100 – 240 VAC ±10%, 50/60 Hz |
Operating Voltage | 24 VDC / 6.67 A |
Temperature tolerance | Storage: 0-40° C Operation: 15-35° C |
Humidity tolerance | 10-80% non-condensing Maximum relative humidity of 80 % for temperatures up to 31 °C; decreasing linearly to 50 %, relative humidity up to 40 °C |
Dimensions (W x D x H) | 334 x 437 x 287 mm |
Weight | 21.8 kg |
Brochure of the Centro and Centro XS microplate luminometers
PDF | 461.4 KB
Download nowMycoplasma is a common contaminant in cell cultures which can compromise the…
EN | PDF | 361.9 KB
Download nowValidation of the Centro LB 963 for the Dual-Luciferase® Reporter System from…
EN | PDF | 300.4 KB
Download nowBrochure of the Centro and Centro XS microplate luminometers
PDF | 461.4 KB
Download nowMycoplasma is a common contaminant in cell cultures which can compromise the…
EN | PDF | 361.9 KB
Download nowValidation of the Centro LB 963 for the Dual-Luciferase® Reporter System from…
EN | PDF | 300.4 KB
Download nowThere are plenty of articles in the scientific literature using the Centro. Here you have a selection of recent papers.
Year | Authors | Journal | Title | Method |
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2023 | Špela Tomaž, Marko Petek, Tjaša Lukan, Karmen Pogačar, Katja Stare, Erica Teixeira Prates, Daniel A Jacobson, Jan Zrimec, Gregor Bajc, Matej Butala, Maruša Pompe Novak, Quentin Dudley, Nicola Patron, Ajda Taler-Verčič, Aleksandra Usenik, Dušan Turk, Salomé Prat, Anna Coll, and Kristina Gruden | Plant Physiology | In planta luciferase transactivation assay | |
2022 | Sarah Temmam, Khamsing Vongphayloth, Eduard Baquero, Sandie Munier, Massimiliano Bonomi, Béatrice Regnault, Bounsavane Douangboubpha, Yasaman Karami, Delphine Chrétien, Daosavanh Sanamxay, Vilakhan Xayaphet, Phetphoumin Paphaphanh, Vincent Lacoste, Somphavanh Somlor, Khaithong Lakeomany, Nothasin Phommavanh, Philippe Pérot, Océane Dehan, Faustine Amara, Flora Donati, Thomas Bigot, Michael Nilges, Félix A. Rey, Sylvie van der Werf, Paul T. Brey & Marc Eloit | Nature | Bat coronaviruses related to SARS-CoV-2 and infectious for human cells | Pseudovirus assays (firefly luciferase) |
2022 | Jianyuan Zhao, Qian Liu,Dongrong Yi, Quanjie Li, SaiSai Guo, Ling Ma, Yongxin Zhang, Dongxin Dong, Fei Guo, Zhenlong Liu, Tao Wei, Xiaoyu Li, and Shan Cena | Antiviral Research | Gaussia Luciferase | |
2021 | Hannah Kinoshita, Jessica Durkee-Shock, Mariah Jensen-Wachspress, Vaishnavi V. Kankate, Haili Lang, Christopher A. Lazarski, Anjeni Keswani, Kathleen C. Webber, Kimberly Montgomery-Recht, Magdalena Walkiewicz, Luigi D. Notarangelo, Peter D. Burbelo, Ivan Fuss, Jeffrey I. Cohen, Catherine M. Bollard, and Michael D. Keller | Journal of Clinical Immunology | Robust Antibody and T Cell Responses to SARS-CoV-2 in Patients with Antibody Deficiency | Luciferase immunoprecipitation |
2021 | Chao Yang, Rui Liu, Jinhuan Pang, Bin Ren, Huanbin Zhou, Gang Wang, Ertao Wang, and Jun Liu | Nature Communications | Oxidative burst and MAPK | |
2021 | Alexandra Rhoden, Felix W. Friedrich, Theresa Brandt, Janice Raabe, Michaela Schweizer, Jana Meisterknecht, Ilka Wittig, Bärbel M. Ulmer, Birgit Klampe, June Uebeler, Angelika Piasecki, Kristina Lorenz, Thomas Eschenhagen, Arne Hansen, and Friederike Cuello | Redox Biology | Lactate-Glo™ | |
2021 | Chunyan Wang, Rien van Haperen, Javier Gutiérrez-Álvarez, Wentao Li, Nisreen M. A. Okba, Irina Albulescu, Ivy Widjaja, Brenda van Dieren, Raul Fernandez-Delgado, Isabel Sola, Daniel L. Hurdiss, Olalekan Daramola, Frank Grosveld, Frank J. M. van Kuppeveld, Bart L. Haagmans, Luis Enjuanes, Dubravka Drabek, and Berend-Jan Bosch | Nature Communications | Pseudotyped virus neutralization assay | |
2021 | Emeline Bonsergent, Eleonora Grisard, Julian Buchrieser, Olivier Schwartz, Clotilde Théry, and Grégory Lavieu | Nature Communications | Extracellular vesicle uptake using NLuc | |
2021 | Silke D. Werle, Julian D. Schwab, Marina Tatura, Sandra Kirchhoff, Robin Szekely, Ramona Diels, Nensi Ikonomi, Bence Sipos, Jan Sperveslage, Thomas M. Gress, Malte Buchholz, and Hans A. Kestler | Cancers | Unraveling the Molecular Tumor-Promoting Regulation of Cofilin-1 in Pancreatic Cancer | Luminescent BrdU assay |
2021 | Seiya Ozono, Yanzhao Zhang, Hirotaka Ode, Kaori Sano, Toong Seng Tan, Kazuo Imai,Kazuyasu Miyoshi, Satoshi Kishigami, Takamasa Ueno, Yasumasa Iwatani, Tadaki Suzuki & Kenzo Tokunaga | Nature Communications | SARS-CoV-2 D614G spike mutation increases entry efficiency with enhanced ACE2-binding affinity | Pseudotyped virus quantification using Nano-Glo® HiBiT Lytic Detection System |
2020 | Maureen Spit, Nicola Fenderico, Ingrid Jordens, Tomasz Radaszkiewicz,Rik GH Lindeboom, Jeroen M Bugter, Alba Cristobal, Lars Ootes, Max van Osch,Eline Janssen, Kim E Boonekamp, Katerina Hanakova, David Potesil, Zbynek Zdrahal,Sylvia F Boj, Jan Paul Medema, Vitezslav Bryja, Bon-Kyoung Koo, Michiel Vermeulen & Madelon M Maurice | The EMBO Journal | RNF43 truncations trap CK1 to drive niche-independent self-renewal in cancer | Dual Luciferase |
2020 | Alexandre Quilbe, Olivier Moralès, Martha Baydoun, Abhishek Kumar, Rami Mustapha, Takashi Murakami, Bertrand Leroux, Clémentine de Schutter, Elise Thecua, Laurine Ziane, Ludovic Colombeau, Céline Frochot, Serge Mordon, and Nadira Delhem | Journal of Clinical Medicine | An Efficient Photodynamic Therapy Treatment for Human Pancreatic Adenocarcinoma | CellTiter-Glo® |
2020 | Sungmi Song, Jin-Young Lee, Ludmila Ermolenko, Aloran Mazumder, Seungwon Ji, Heeju Ryu, HyeJin Kim, Dong-Wook Kim, Jung Weon Lee, Mario Dicato, Christo Christov, Michael Schnekenburger, Claudia Cerella, Déborah Gérard, Barbora Orlikova-Boyer, Ali Al-Mourabit and Marc Diederich | International Journal of Molecular Sciences | GSH/GSSG-Glo™ | |
2020 | Urooj Fatima, Zhenyu Zhang, Haili Zhang, Xue-Feng Wang, Ling Xu, Xiaoyu Chu,Shuang Ji and Xiaojun Wang | Viruses | Dual Luciferase | |
2020 | Stefan Reich, Chi D. L. Nguyen, Canan Has, Sascha Steltgens, Himanshu Soni, Cristina Coman,Moritz Freyberg, Anna Bichler, Nicole Seifert, Dominik Conrad, Christiane B. Knobbe-Thomsen,Björn Tews, Grischa Toedt, Robert Ahrends & Jan Medenbach | Nature Communications | NADP/NADPH-Glo™ | |
2020 | Yeojung Nam, Kyung Min Kang, Se Ra Sung, Ji Eun Park, Yun-Jeong Shin, Seung Hun Song, Ju Tae Seo, Tae Ki Yoon, Sung Han Shim | Asian Journal of Andrology | Dual Luciferase | |
2020 | Monica Z. Wu, Haruichi Asahara, George Tzertzinis, and Bijoyita Roy | RNA | Synthesis of low immunogenicity RNA with high-temperature in vitro transcription | BioLux® Cypridina Luciferase Assay |
2020 | Da Hee Lee, Na Eun Kwon, Won-Ji Lee, Moo-Seung Lee, Doo-Jin Kim, Ji Hyung Kim and Sung-Kyun Park | Cells | Increased O-GlcNAcylation of c-Myc Promotes Pre-BCell Proliferation | Glucose Uptake-Glo™ |
2020 | Qingkang Lyu, Irene S. Ludwig, Peter J. S. Kooten, Alice J. A. M. Sijts, Victor P. M. G. Rutten, Willem van Eden, Femke Broere | Cell Stress and Chaperones | Steady-Glo® | |
2020 | Daichi Hayashi, Toshiharu Shirai, Ryu Terauchi, Shinji Tsuchida, Naoki Mizoshiri, Yuki Mori, Yuji Arai, Osam Mazda, and Toshikazu Kubo | Oncology Letters | Pristimerin inhibits the proliferation of HT1080 fibrosarcoma cells by inducing apoptosis | RealTime-Glo™ MT Cell Viability Assay |
2020 | Stefano Del Prato, Jahoon Kang, Michael E. Trautmann, John Stewart, Christopher H. Sorli, Michael Derwahl, Alfonso Soto and Kun‐Ho Yoon | Diabetes, Obesity and Metabolism | cAMP-Glo™ | |
2020 | Vito Lampasona, Massimiliano Secchi, Marina Scavini, Elena Bazzigaluppi, Cristina Brigatti, Ilaria Marzinotto, Alberto Davalli, Amelia Caretto, Andrea Laurenzi, Sabina Martinenghi, Chiara Molinari, Giordano Vitali, Luigi Di Filippo, Alessia Mercalli, Raffaella Melzi, Cristina Tresoldi, Patrizia Rovere-Querini, Giovanni Landoni, Fabio Ciceri, Emanuele Bosi and Lorenzo Piemonti | Diabetologia | LIPS with NanoLuc® Luciferase | |
2019 | Jun Weng, Xu Han, Kaiyu Liu, Jiong Yang, Shiruo Wei, Yue Zhang, Fanhong Zeng, Yang Li, Li Shen, Yi Gao | International Journal of Biological Sciences | Dual-Glo® |
You can find additional publications which have used Centro instruments in publication databases.
Please keep in mind that the list provided is not exhaustive, but it offers valuable examples of experiments performed with our instruments and published in peer-reviewed journals.
MycoAlert® is a trademark by Lonza Group.
Picture of the mycoplasma in the Mycoalert application note created by Martina Maritan, Scripps Research, adapted from https://ccsb.scripps.edu/gallery/mycoplasma_model/ under the Creative Commons Attribution International 4.0 license.