Glossary Bioanalytics

ATP measurements are used to monitor substances and surfaces for bacterial contamination, as well as for waste water analysis.

AlphaScreen® relies on hydrogel coated Donor and Acceptor beads providing functional groups for conjugation to biomolecules.

AquaSpark™ is a new generation of chemiluminescent probes based on dioxetane derivatives that exhibit enhanced properties over traditional dioxetanes.

BRET (Bioluminescence Resonance Energy Transfer) is based on the fact that the energy derived from a luciferase reaction can be used to excite a fluorescent protein if the latter is in close proximity to the luciferase enzyme.

CALUX® Assays can be used to detect dioxins, dioxin-like PCB's, (anti)estrogens and (anti)androgen compounds, and other chemicals, by using a luminometer.

The activity of caspases can be monitored using luminescence, fluorescence intensity or TRF, and is a key method in apoptosis research.

Cellular chemiluminescence (CL) is the production of light by neutrophil granulocytes, monocytes or macrophages when phagocytosis is activated.

Coating means the immobilization of antigen, antibodies or any other compound on the well surface, for the purpose of a binding assay. The „coated plate“ is part of a commercial ELISA kit, or of a home-brew (self-made) ELISA assay.

Fluorescence polarisation (FP) is the ideal tool to measure the binding of a small molecule to a much larger one.

FRET (Fluorescence Resonance Energy Transfer) can be performed in a microplate reader to detect the interactions of proteins and nucleic acids.